The Morphology of Simian Immunodeficiency Virus as Shown by Negative Staining Electron Microscopy

Morphology and structure of lipoproteins revealed by an optimized negative-staining protocol of electron microscopy[S]

Plasma lipoprotein levels are predictors of risk for coronary artery disease. Lipoprotein structure-function relationships provide important clues that help identify the role of lipoproteins in cardiovascular disease. The compositional and conformational heterogeneity of lipoproteins are major barriers to the identification of their structures, as discovered using traditional approaches. Althou...

Electron Microscopy of Fowl Pox Virus

Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.

A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.

Virus-Induced IL-2 Expression by Central Virus/Simian Human Immunodeficiency Dysfunction of Simian Immunodeficiency

Optimized negative-staining electron microscopy for lipoprotein studies.

BACKGROUND Negative-staining (NS), a rapid, simple and conventional technique of electron microscopy (EM), has been commonly used to initially study the morphology and structure of proteins for half a century. Certain NS protocols however can cause artifacts, especially for structurally flexible or lipid-related proteins, such as lipoproteins. Lipoproteins were often observed in the form of rou...